Mechanisms of
Sperm Motility:
Dr. Charles Lindemann
Dr. Lindemann's Former Labrats
Robin Autore-Blalock

Our "Queen Lab Rat", Robin our "Lab Slave". For over 8 years, this poor soul was responsible for cleaning up our messes and making reagents, all the while keeping the computer and computer users happy (most of the time a thankless job). She is a "jack of all trades"; whatever we needed done, that we couldn't or wouldn't do, we just called on Robin to help. Unfortunately, we can no longer exploit her talents since Robin is now employed full-time at William Beaumont Hospital in Royal Oak, Michigan where she is putting her Master's Degree in Physical Therapy (April 2001) to good use. Robin's contributions on the bull sperm force & ADP study were significant, as a result she was a co-authored in Molecular Biology of the Cell 12:443a.

 

Benjamin Dionne

 

Khrystyne Clifton

Khyrstyne worked in the laboratory for a short time but accomplished much. She kept the lab and glassware clean. More importantly, she force calibrated a bunch of glass microprobes for our use. It is not a glamorous job, but it is very important to our work. Her efforts will assist us for some time to come; we've not yet broken every probe she calibrated. Khrystyne moved to Indiana to pursue studies at Purdue. We wish her much success in her new adventure.

Dervina Gjysma

Dervina started in the lab as an assistant for Kathie. She was to help with general lab duties and administrative paperwork. We ended up utilizing her talents for research. She was responsible for the maintenance of our Chlamydomonas stock cultures. Dervina graduated in December, 2011 with her Bachelor's Degree. She is pursuing a career in medicine with her sights set on medical school.

Dana Holcomb

Dana Holcomb finished the thesis requirements for her Master's of Science in Biological Sciences, a degree she was awarded April 2001. She conducted research in our laboratory for 6 years, earning herself the title "Princess Lab Rat". Her lab research efforts as an undergraduate student determined that there is a critical t-force threshold required for normal, continuous, flagellar beating as published in Cell Motility and the Cytoskeleton: 44:177-189. She also contributed to the bull force measurement study published in Biophysical Journal 79:468-478. Her graduate research project was to find the passive stiffness of a bull sperm, which is really fun stuff folks! She is now the Field Application Specialist for Diagnostic Instruments, manufacturer of SPOT cameras for digital microscopy, in Sterling Heights, Michigan.

Courtney Kelsch

Courtney started working in our lab in May, 2006 as a result of her receiving the Undergraduate Student Research Summer Fellowship in Biological Sciences at Oakland University. Originally, she worked on the chemotactic responses of mammalian sperm as part of her fellowship project. Although this was not a specific aim of the project, the program benefited greatly by her inclusion in the lab. She successfully worked out the details of mammalian sperm hyperactivation and the production of optimized in-vitro fertilization medium for our use with rodent sperm. Furthermore, she provided important and much needed assistance to Kathie on every project in the lab during her tenure. Courtney graduated in April, 2008 but continued to work with us until she left for Miami, FL to pursue graduate studies. Courtney was very reliable and detail-oriented. Her contributions resulted in coauthorship on two papers published in Biology of Reproduction

Anetra Knowles

Anetra joined the laboratory in January, 2006. Initially, she was assigned the important task of cleaning and organizing the lab. Anetra also assisted Dr. Lindemann with graphing Geometric Clutch computer model output data. She assisted Kathie with studies of the response to calcium in mouse sperm and the effect of calcium on microtubule doublet sliding. She graduated August 2007, with a Bachelor of Science degree.

Anissa Knowles

Anissa joined us in January, 2006 at the same time as her twin sister, Anetra. Her job was to assist Kathie in quantifying the results from the microtubule sliding and response to calcium work with sperm from SPAG16L mice, graciously supplied to us by Drs. Zhibing Zhang and Jerome F. Strauss III from Virginia Commonwealth University. Her help allowed us to present an abstract at the 2006 ASCB Annual Meeting. Anissa graduated with a Bachelor of Science degree in April, 2007.

Laurel Lindemann

Laurel worked on the project during the summer of 2007. As one may surmise from the last name, she is related to Dr. Lindemann. She is an undergraduate student at Michigan State University majoring in Zoology and Japanese. Her primary function in the lab was to assist Kathie. As a result she was given the important taks in the lab that have a tendency to be shoved to the back burner when Kathie has too many tasks to perform at once. On occasion she assisted with preparation for experiments. Her largest accomplishment of the summer was to update the database of research articles important to our field of study which required many hours of pretty much mundane work. Although we would have welcomed Laurel back to the lab this summer, she chose to pursue other opportunities.

Elizabeth Long

Elizabeth worked on chillin' bull sperm to determine how the beat changes as a function of decreasing temperature. She was an Honor's College student and this was her senior project for the program. In August 2006, Elizabeth completed her internship at St. John's Hospital. This was the final phase of the program in Medical Laboratory Sciences here at OU. She is employed full time with St. John Hospital but has taken a 3 month leave of absence to pursue missionary work in Africa. She is utilizing her training and education in a way to help others, which was a goal she set for herself long ago. We can't wait for her to report back about her travels and experiences.

Kaja Lund

Kaja Lund, a Norwegian import and former member of OU's soccer team, completed a boatload of curvature and shear angle measurements on sperm cells for us. She received her BS degree with a major in Biological Science in April 2001. She worked in the our lab until December 2001 when she returned home to Norway. Kaja is currently a "Candidatus Scientific", which is a Master of Science, but it takes a little bit longer. She is working with mitogen activated protein kinases and their role in apoptosis in prostate cancer cells, or more specific, the role of c-jun n-terminal kinase in apoptosis induced by phorphol ester and thapsigargin in the androgen-sensitive prostate cancer cell line LNCaP.

Lisa Macauley

Lisa worked on several different projects in the lab. One of her more important contributions was to work out the best method to reactivate Triton X100-extracted mouse sperm such that they exhibit motility more aligned with the motility of live mouse sperm. She also contributed a great deal to the work on the counter-bend phenomenon in dynein-disabled rat sperm. As a result she is a co-author on the report of our results that was published in Biophysical Journal: 89:1165-74. last year.

Mark Moritz

Mark "Superstar" Moritz was a Howard Hughes Undergraduate Research Fellowship recipient who worked in our lab for 2 years. His work examined the calcium hook in rat sperm and was published in Cell Motility and the Cytoskeleton 49:33-40. Mark was dedicated to many extra-curricular activities, both on and off campus.

Danial Oberski

Danial Oberski was also known as "Chlamy Dan" or "Crazy Dan". For 2 1/2 yrs Danial worked with Chlamydomonas and tested the Geometric Clutch hypothesis in that model system. He also contributed to the bull force measurement study published in Biophysical Journal 79:468-478. Danial received his State of Michigan Teaching Certificate from Ferris State University and is currently the Director of the Program for Expelled Students of Traverse City Area Public Schools. He is also an Adjunct Professor at Northwestern Michigan College teaching Biology. It makes us happy to know that Dan is teaching. We are confident that the enthuasiasm he has for science and education is evident in his lectures.

Brian Parys

Brian joined the ranks in April 2003 as the first non-science undergraduate to work on our project. He is responsible for creating a computer program that allows us to do more sensitive and accurate force measurements with our current imaging/manipulation system. The programming is nearing completion. Brian is currently employed full time by DTE Energy as a Distributions Operations Technician. In April 2004, he graduated with a degree in Electrical Engineering.

Dominic Pelle

Dominic contributed to several different research projects while working in our lab. In addition to looking at the effect of ADP concentration on reactivated bull sperm motility, he has measured the passive stiffness of sea urchin sperm and ADP-treated bull sperm. He also did work that involved further evaluation of the counter-bend phenomenon in rat sperm and sea urchin sperm. Dominic is co-author on several abstracts and two papers, a testament to his contributions to the program.

Kristen Ponichter

Kristen joined our program in May 2004 and graduated in June 2005 with a Bachelor of Science degree. Her project was to analyze sliding/disintegration patterns in Ca2+-treated normal and mutant mouse sperm. In order to carry out her study she was required to refine a method for microtubule extraction/sliding from mouse sperm, which she did very well. Kristen graduated from the University of Pittsburg's School of Dental Medicine in 2009.

Daniel Walton

As the funniest of the group, Daniel kept us laughing and smiling in his time in the lab. He spent most of his time updating and maintaining the website and computer systems. Daniel considers himself an expert in Indolence as well as an avid procrastinator. Daniel plans to get his degree in "something" whenever the time feels right. Often disinclined to adhere to premature planning, he'd be more apathetic if he weren't so lethargic.

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