Promoters Containing Artificially-made "Pre-melted" Regions

A very useful tool in the analysis of the biochemical mechanisms of Transcription Initiation and Promoter Escape are specially made templates that contain a region of 10-20 bases in which the nucleotides of the upper and lower strands do not match for base-pairing. This creates an artificial “bubble” that alleviates the requirement for the TFIIH helicase at carious steps of early transcription. The opening in the Ad(-9/-1) is sufficient to allow Transcription Initiation in the absence of TFIIH. The Ad(-9/+9) has an opening sufficient to allow both Transcription Initiation and Promoter Escape in the absence of TFIIH.

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