Arik Dvir - Biochemistry
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ARIK DVIR
Ph.D. in Biochemistry, Hebrew University, Jerusalem, 1990

Associate Professor.
Department of Biological Sciences.

Coordinator, The Biochemistry Program.
College of Arts and Sciences.

COURSES

Arik Dvir

RESEARCH: Biochemistry and Enzymology of Eukaryotic Gene Transcription

Humans have an estimated 40,000 to 60,000 genes in every cell, but not the same genes are active in each cell. At any given time, only about 1-2% of the genes are active. The process of gene activation is called Gene Expression. Molecular control mechanisms that determine which genes are expressed are critical to normal growth, development and cell function.

intro1

The first step in gene expression is transcription. For protein-coding genes, this process is catalyzed by the enzyme RNA polymerase II. Regulation of gene expression is essential if the cell is to make optimal use of available use of available energy. In multicellular organisms, gene expression is also an essential tool for determining the differentiation between various tissues. Much of the way cells regulate which genes are to be expressed has to do with controlling RNA polymerase II, and by that, transcription. Therefore, transcription is key to understanding development, homeostasis, and disease.

PUBLICATIONS:

  1. W.J. Moon, J.A. Apostol, A.J. McBride, L.I. Shukla, A .Dvir, and Z.F. Burton (2004) "Efficient production of recombinant human transcription factor IIE" Protein Expr. Purif. 34:317-23

  2. A. Dvir, J.W. Conaway, and R.C. Conaway (2003) "Assays for Investigating the Mechanism of Promoter Escape by RNA Polymerase II" Methods Enzymol. 370:733-40.

  3. X. Wang, L. Spangler, and A. Dvir (2003) “Promoter escape by RNA polymerase II: Downstream promoter DNA is continuously required during transcription initiation and promoter escape”. Journal of Biological Chemistry 278:10250-10256.

  4. A. Dvir (2002) "Promoter Escape by RNA polymerase II", Biochimica et Biophysica Acta, 1577(2):208-223.

  5. L. Spangler, X. Wang, J.W. Conaway, R.C. Conaway, and A. Dvir (2001) "TFIIH action in transcription initiation and promoter escape requires distinct regions of downstream promoter DNA". Proceedings of the National Academy of Sciences (USA), 98:5544-5549.

  6. A. Dvir, J.W. Conaway, and R.C. Conaway (2001) "Mechanism of transcription initiation and promoter escape by RNA polymerase II". Current opinions in Genetics and Development, 11:209-214.

  7. J. W. Conaway, A. Shilatifard, A. Dvir, and R.C. Conaway (2000), "Control of Elongation by RNA Polymerase II". Trends in Biochemical Sciences, 25:375-380.

  8. J. W. Conaway, A. Dvir, R.J. Moreland, Q. Yan, B.J. Elmendorf, S. Tan, and R.C. Conaway (1998), "Mechanism of Promoter Escape by RNA Polymerase II". Cold Spring Harbor Symposia on Quantitative Biology, 63:357-364.

  9. A. Dvir, S. Tan, J. W. Conaway, and R. C. Conaway (1997), "Promoter escape by RNA polymerase II: Formation of an escape-competent transcriptional intermediate is a prerequisite for exit of polymerase from the promoter". Journal of Biological Chemistry, 272:28175-28178.

  10. A. Dvir, R.C. Conaway, and J.W. Conaway (1997), "A role for TFIIH in controlling the activity of early RNA polymerase II elongation complexes". Proceedings of the National Academy of Sciences (USA), 94:9006-9010.

  11. D. Reines, A. Dvir, J.W. Conaway, and R.C. Conaway (1997), "Assays for investigating transcription by RNA polymerase II in vitro". Methods, 12(3):192-202.

  12. A. Dvir, R.C. Conaway, and J.W. Conaway (1996), "Promoter escape by RNA polymerase II: a role for an ATP cofactor in suppression of arrest by polymerase at promoter-proximal sites". Journal of Biological Chemistry, 271:23352-23356.

  13. A. Dvir, K.P. Garret, C. Chalut, J.-M. Egly, J.W. Conaway, and R.C. Conaway (1996), "A role for ATP and TFIIH in activation of the RNA polymerase II preinitiation complex prior to transcription initiation". Journal of Biological Chemistry, 271:7245-7248.

  14. S.R. Peterson, A.S. Jesch, T.N. Chamberlin, A. Dvir, S.K. Rabindran, K. Wu, and W.S. Dynan (1995), "Stimulation of the DNA-dependent protein kinase by RNA polymerase II transcriptional activator proteins". Journal of Biological Chemistry, 270:1449-1454.

  15. A. Dvir, L. Y. Stein, B. L. Calore, and W. S. Dynan (1993), "Purification and characterization of a template-associated protein kinase that phosphorylates RNA polymerase II" Journal of Biological Chemistry, 268:10440-10447.

  16. A. Dvir, S. R. Peterson, M. Knuth, H. Lu, and W. S. Dynan (1992), "Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II" Proceedings of the National Academy of Sciences (USA), 89:11920-11924.

  17. S. R. Peterson, A. Dvir, C. W. Anderson, and W. S. Dynan (1992), "DNA binding provides a signal for phosphorylation of the RNA polymerase II heptapeptide repeats" Genes and Development, 6: 426-438.

  18. A. Dvir, Y. Milner, O. Chomsky, C. Gilon, A. Gazitt, and A. Levitzki (1991), "The inhibition of EGF-dependent proliferation of human keratinocytes by tyrphostins tyrosine kinase blockers" Journal of Cell Biology, 113: 857-865.

  19. A. J. Simon, Y. Milner, S. P. Saville, A. Dvir, D. Mochly-Rosen, and E. Orr (1991), "The identification and purification of a mammalian-like protein kinase C in the yeast Saccharomyces cerevisiae" Proceedings of the Royal Society of London. Series B. Biological Sciences 243:165-171.


Comments? Questions? Contact Dr. Dvir.